Gel Electrophoresis
Your first step in your investigation is to look for certain genetic mutations in your patient.
You will be testing the patient's DNA for PKU, sickle cell anemia, and cystic fibrosis.
You will load the patient's DNA sample along with the 3 genetic mutation samples in the gel electrophoresis machine. You will apply an electric current to the samples and they will seperate based on DNA fragment length. The shortest will move the farthest and the longest will move the least. You will then compare the banding patterns and see if there are any matches. If there are NOT it means your patient does not have or carrier one of those genetic mutations.
Be sure to wear your goggles.
Directions
Gel Electrophoresis
You will be testing the patient's DNA for PKU, sickle cell anemia, and cystic fibrosis.
You will load the patient's DNA sample along with the 3 genetic mutation samples in the gel electrophoresis machine. You will apply an electric current to the samples and they will seperate based on DNA fragment length. The shortest will move the farthest and the longest will move the least. You will then compare the banding patterns and see if there are any matches. If there are NOT it means your patient does not have or carrier one of those genetic mutations.
Be sure to wear your goggles.
Directions
Gel Electrophoresis
- Pour 12 ml of buffer solution over the gel.
- Record the order you will load the gels. A picture might be helpful.
- Load the DNA samples from your evidence kit into the gel. Be sure to use a clean micro-pipette tip between each sample so you don’t cross contaminate.
- Place the gel into the electrophoresis machine.
Analysis Questions...
1. Did any of the banding patterns match?
2. If so, which ones?
3. Is the genetic sequence normal or mutated and how do you know?
Reference Resources Below:
1. Did any of the banding patterns match?
2. If so, which ones?
3. Is the genetic sequence normal or mutated and how do you know?
Reference Resources Below: